Fig 7. Regulation of Mcm21 desumoylation during the cell cycle and in response to DNA replication stress.

A) Tetrad dissection of ulp2-707 and ulp2-SIM^3ACCR^3A. B) Plating assay to evaluate the growth of various ulp2 mutants in response to high temperature, HU and benomyl. As controls, the rad53Δ mutant shows an expected hypersensitivity to HU, while the mad1Δ mutant shows an expected hypersensitivity to benomyl. C-D) Sumoylation of Mcm21 in wild-type and the ulp2-SIM^3ACCR^3A mutant strains during different stages of the cell cycle, which are confirmed by FACS analysis. E-F) Effect of HU treatment on sumoylated Mcm21 in wild-type and the ulp2-SIM^3ACCR^3A mutant. G) Effect of ndc10-1 on sumoylated Mcm21 in the wild-type strain background. Both wild type and ndc10-1 mutant cells were grown at room temperature, shifted to 37 °C for 6 hours, and then the cells were collected for FACS and Ulp1-C580S pull-down experiments.