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. 2019 Nov 20;15(11):e1008497. doi: 10.1371/journal.pgen.1008497

Fig 2. Systematic mutational analysis of LPS genes in G27.

Fig 2

LPS samples from G27 wild-type and isogenic LPS mutants were analysed by silver staining (upper panel), and Western blot using anti-Lex (middle panel) and anti-Ley antibodies (lower panel). (A): Lane 1: G27 wild-type full-length LPS expressing both Lex and Ley; Lane 2: the Hep II transferase mutant ΔHP1191; Lane 3–5: the O-antigen initiating enzyme (WecA), flippase (Wzk), and ligase (WaaL) mutants; Lane 6: the new glycosytransferase gene mutant ΔHP0102; Lane 7: the Trio Hep transferase mutant ΔHP0479; Lane 8: the glucan transferase mutant ΔHP0159; (B): Lane 1–2: G27 wild-type and ΔHP0159; Lane 3–4: the new glycosytransferase gene mutants ΔHP1283 and ΔHP1578; Lane 5–6: the poly-LacNAc Gal and GlcNAc transferase mutants ΔHP0826 and ΔHP1105; Lane 7: the new glycosytransferase gene mutant ΔHPG27_1230; Lane 8: G27 wild-type; (C): Lane 1–9: G27 wild-type, the Hep III transferase mutant ΔHP1284, ΔfutA, ΔfutB, ΔfutC, ΔHP1416, ΔHP0208, ΔHP0619, and the new glycosyltransferase mutant ΔHP0805; (D): Lane 1, G27 wild-type; Lane 2–3, ΔHP0102 and ΔHP0102 complementation; Lane 4–5, ΔHP1283 and ΔHP1283 complementation.