Table 1. CAZy family assignment of H. pylori glycosyltransferase genes.
NO. | GT family | 26695 ORF | G27 ORF | Activity | Function | Mutatagenesis in this study | Reference(s) |
---|---|---|---|---|---|---|---|
1. | GT-2 | HP0102 | HPG27_94 | Putative glycosyltransferase | Unknown | √ | this study |
2. | GT-8 | HP1578 | HPG27_1515 | Putative glycosyltransferase | Unknown | √ | this study |
3. | GT-9 | ※ | HPG27_1229 | Putative glycosyltransferase | Unknown | this study | |
4. | GT-9 | ※ | HPG27_1230 | Putative glycosyltransferase | Unknown | √ | this study |
5. | GT-25 | HP0805 | HPG27_761 | Putative glycosyltransferase | Unknown | √ | this study |
6. | GT-9 | HP1283 | HPG27_1235 | Putative heptosyltransferase# | Heptan# | √ | this study and [22] |
7. | GT-19 | HP0867 | HPG27_821 | LpxB |
Lipid A |
[10] | |
8. | GT-30 | HP0957 | HPG27_905 | WaaA | [10] | ||
9. | GT-9 | HP0279 | HPG27_258 | Hep I transferase | Core-oligosaccharide | √ | [6] |
10. | GT-9 | HP1191 | HPG27_1136 | Hep II transferase | √ | [6] | |
11. | GT-9 | HP1284 | HPG27_1236 | Hep III transferase | √ | [6] | |
12. | GT-8 | HP1416 | HPG27_ 1339 | α-1,2/4 Glc transferase | √ | [23] | |
13. | GT-9 | HP0479 | HPG27_437 | α-1,2-DD-Hep transferase | Trio | √ | [24,25] |
14. | GT-8 | HP0159 | HPG27_146 | α-1,6 Glc transferase | Glucan | √ | [23] |
15. | GT-8 | HP1105 | HPG27_1046 | β-1,3-GlcNAc transferase | Lewis antigen | √ | [26] |
16. | GT-25 | HP0619 (JHP0562/3) | HPG27_579/580 | β-1,3-Gal transferase | √ | [27–29] | |
17. | GT-25 | HP0826 | HPG27_785 | β-1,4-Gal transferase | √ | [30] | |
18. | GT-10 | HP0379 | HPG27_613 | FutA | √ | [31] | |
19. | GT-10 | HP0651 | HPG27_1018 | FutB | √ | [31] | |
20. | GT-11 | HP0093/0094 | HPG27_86 | FutC | √ | [32] | |
21. | GT-8 | HP0208 | HPG27_190 | Putative glycosyltransferase | LPS biosynthesis ? | √ | [26,33] |
22. | GT-4 | HP0421 | HPG27_952 | Cholesterol α-glucosyltransferase | Not associated with LPS biosynthesis | [34] | |
23. | GT-28 | HP1155 | HPG27_1099 | MurG | [10] | ||
24. | GT-51 | HP0597 | HPG27_557 | PBP-1A | [10] |
※ partial fragment or homologue not found in this strain
# HP1283 function was unknown at the time of this study but has recently been annotated [22]