Skip to main content
. Author manuscript; available in PMC: 2020 Nov 19.
Published in final edited form as: Immunity. 2019 Nov 12;51(5):899–914.e7. doi: 10.1016/j.immuni.2019.10.010

Figure 6. Macrophages protect the heart in sepsis.

Figure 6.

(A) pHrodo E.coli in cardiac macrophages. (B) Propidium Iodide (PI) in heart macrophages after CLP. Control n=12, CLP n=4 per time point. (C) Flow plots and (D) number of cardiac macrophages in Csf1+/+ and Csf−/− mice. (E) Serum troponin levels in naive and CLP Csf1+/+ and Csf−/− mice. Naive Csf1+/+ n=6, Csf1−/− n=3, CLP Csf1+/+ n=6, Csf1−/− n=9. (F) Top regulated genes after CLP in heart macrophages. (G) Protein-protein association network for the top 100 genes by p-value that are differentially expressed in cardiac macrophages after CLP. (H) Serum troponin levels in Cx3cr1 Il10+/+ and Cx3cr1 Il10−/− mice after CLP. Naive Cx3cr1 Il10+/+ n=6, Cx3cr1 Il10−/− n=4, CLP Cx3cr1 Il10+/+ n=6, Cx3cr1 Il10−/− n=4. (I) Apoptotic cardiomyocytes by TUNEL staining. n=3 mice per group. (J) TUNEL+ myocyte. Dashed line marks cell border. (K) Outline for L and M. (L) MHCII expression in cardiac macrophages. Naive Cx3cr1 Il10+/+ n=6, Cx3cr1 Il10+/+ CLP n=6, Cx3cr1 Il10−/− CLP n=7 biological replicates. (M) qPCR in sorted cardiac macrophages. Naive Cx3cr1 Il10+/+ n=2–5, Cx3cr1 Il10+/+ CLP n=8, Cx3cr1 Il10−/− CLP n=5–6 biological replicates. (N) Gene expression in local versus recruited cardiac macrophages isolated from Cx3cr1CreER/+R26tdTomato/+ mice, n=4 biological replicates for control local, n=5 control recruited, n=10 CLP d4 local, n=8 CLP d4 recruited. Data are mean ± s.e.m. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Unpaired t-test or ordinary one-way ANOVA with Dunnett’s multiple comparison test was used for statistical analysis. See also Figure S6.

HHS Vulnerability Disclosure