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. 2019 Nov 18;8:e48622. doi: 10.7554/eLife.48622

Figure 12. Rebound activity after photoinhibition.

Figure 12.

(A) Mean PSTH of pyramidal neurons during photoinhibition in VGAT-ChR2-EYFP mice. Dashed lines, photostimulus onset and offset. All neurons within 0.4 mm from the laser center across all cortical depths (n = 78). Data from motor cortex. Laser power, 7 mW. Spike rates were analyzed in three time windows before, during, and after photostimulation. Photostimulus duration, 1 s. (B) Spike rate of pyramidal neurons before, during, and after photostimulation for different laser powers and photostimulation durations. Mean ± s.e.m. across neurons, bootstrap. (C) Rebound activity as a function of laser powers and photostimulation durations. Relative activity is the after-photostimulation spike rate increase from the baseline, normalized to the baseline spike rate (Materials and methods). (D) Rebound activity as a function of photoinhibition strength. Percent of spikes silenced was relative to the baseline spike rate. Data from all mouse lines. VGAT-ChR2-EYFP, barrel cortex, n = 111; PV-IRES-Cre x Ai32, barrel cortex, n = 16; PV-IRES-Cre x ReaChR, barrel cortex, n = 82; Sst-IRES-Cre x Ai32, barrel cortex, n = 65; Emx1-IRES-Cre x Ai35D, barrel cortex, n = 26; VGAT-ChR2-EYFP, ALM, n = 96; PV-IRES-Cre x ReaChR, ALM, n = 129.