| Antibodies |
|
(Clone) |
| IFNγ |
BioLegend |
XGM1.2 |
| IL-17 |
BioLegend |
TC11-18H10.1 |
| IL-13 |
eBioscience |
eBio13A |
| TNFα |
BioLegend |
MP6-XT22 |
| MHCII |
BD Biosciences |
11-5.2 |
| CD86 |
BioLegend |
GL-1 |
| CD206 |
BioLegend |
C068C2 |
| Gal3 |
BioLegend |
eBioM3/38 |
| H3K4me3 |
Active Motif |
MABI 0304 |
| CD45 |
BioLegend |
30-F11 |
| CD3ε |
BioLegend |
145-2C11 |
| CD19 |
BioLegend |
6D5 |
| CD11b |
BioLegend |
M1/70 |
| CD11c |
BioLegend |
N418 |
| Ly6C |
BioLegend |
HK1.4 |
| Ly6G |
BioLegend |
1A8 |
| Lin (CD3/Gr1/CD11b/B220/Ter119) |
BioLegend |
N/A |
| SCA-1 |
BioLegend |
D7 |
| Flt-3 |
BioLegend |
A2F10 |
| c-kit |
BioLegend |
2B8 |
| CD115 |
BioLegend |
AFS98 |
| CD4 |
BioLegend |
GK1.5 |
| CD8 |
BioLegend |
53-6.7 |
| Fizz1 |
R&D Systems |
226033 |
| TNFRI |
BioLegend |
55R-170 |
| TNFRII |
BioLegend |
TR75-32.4 |
| Anti-goat Alexa Fluor 488 |
Abcam |
Catalog no. ab150129 |
| Anti-rabbit Alexa Fluor 594 |
Abcam |
Catalog no. ab150076 |
| Fungal strain |
| C. neo strain 52D |
American Type Culture Collection |
24067 |
| Chemicals, peptides, and recombinant proteins |
| TNFα recombinant antibody |
Leinco |
Clone TN3-19.12 |
| MM102 (MLL1 inhibitor) |
Tocris |
5307 |
| TNFα |
PeproTech |
315-01A |
| IFNγ |
Sigma-Aldrich |
I4777-.1MG |
| IL-4 |
PeproTech |
214-14 |
| Critical commercial assays |
| PE-Cy7 conjugation kit |
Abcam |
ab102903 |
| APC-Cy7 conjugation kit |
Abcam |
ab102859 |
| LEGENDplex mouse inflammation panel |
BioLegend |
740150 |
| LEGENDplex mouse Th cytokine panel |
BioLegend |
740005 |
| RT2 profiler PCR array mouse TH cell differentiation |
Qiagen |
PAMM-503Z |
| Foxp3 Transcription Factor Staining Buffer Kit |
Thermo Fisher Scientific |
A25866A |
| Experimental models: Organisms/strains |
| CBA/J mice |
Jackson Laboratory |
000656 |
| OT-II mice |
Jackson Laboratory |
004194 |
| Oligonucleotides |
Sense primer |
Antisense primer |
| 18S
|
GAGGCCCTGTAATTGGAATGAG |
GCAGCAACTTTAATATCCGCTATTGG |
| iNOS |
TTTGCTTCCATGCTAATGCGAAAG |
GCTCTGTTGAGGTCTAAAGGCTCCG |
| Arginase |
CAGAAGAATGGAAGAGTCAG |
CAGATATGCAGGGAGTCACC |
| Fizz1 |
GGTCCCAGTGCATATGGATGAGACCATAGA |
CACCTCTTCACTCGAGGGACAGTTGGCAGC |
| MHCII |
GCGACGTGGGCGAGTACC |
CATTCCGGAACCAGCGCA |
| CD206 |
CTCTGTTCAGCTATTGGACGC |
CGGAATTTCTGGGATTCAGCTTC |
| IL-12b |
GGAAGCACGGGGAGCAGAATA |
AACTTGAGGGAGAAGTAGGAATGG |
| IL-13 |
GCCAGCCCACAGTTCTACAGC |
GAGATGTTGCTCAGCTCCTCA |
| iNOS (promoter) |
TCCCTAGTGAGTCCCAGTTTTGA |
CTGGTCGCCCGTCCAAGG |
| IL-12b (promoter) |
TTCCCCCAGAATGTTTTGACA |
TGATGGAAACCCAAAGTAGAAACTG |
| β-Actin (promoter) |
AAGGACTCCTATGTGGGTGACGA |
ATCTTCTCCATGTCGTCCCAGTTC |
| Fizz1 (promoter) |
TGCAATTCTTTGATGCTGTGTCT |
AGCACCCTCAACCCAAAGTG |
| KDM5c |
GACCCATCGCCGAGAAGTC |
TCGGGGAGTAAACCTGAAGTT |
| MLL1 |
ATCCTCTCAGACCCATCTGTGT |
GTAGGAGGTCTTCCTCTCTTC |
| Software and algorithms |
| Microsoft Excel |
|
|
| GraphPad Prism v6 |
|
|
| MetaMorph |
|
|
| FlowJo v7 |
|
|
| FACSDiva |
|
|
