FIGURE 2.

Enzymatic MR (E-MR) and nitrogen starvation both extend CLS, dependent on autophagy, and are epistatic. (A) Wild-type cells containing a plasmid that constitutively expresses methionine gamma lyase (i.e., E-MR) are long-lived compared with empty vector-containing control cells. (B) Nitrogen starvation (NS) extends CLS and is epistatic with E-MR. Extension of CLS by (C) E-MR and (D) nitrogen starvation is abrogated in autophagy-deficient (atg5Δ) cells. Also, autophagy-deficient cells simultaneously undergoing both E-MR and nitrogen starvation fail to receive the benefit of either intervention to CLS. Bars denote SEM.