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. 2019 Dec 4;10:5522. doi: 10.1038/s41467-019-13381-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Schematics of mESC culturing, release of spheroids, and expression of germ layer markers in replated aggregates. b Bright-field images of mESC spheroids before and after release from S100 culture materials and formation of differentiating colonies, observed via the outgrowth of cells in the periphery. c Representative fluorescence images of immunostained markers for germ layers in the colonies after 14 days.