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. 2019 Dec 4;9:18363. doi: 10.1038/s41598-019-54978-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Atrazine metabolic degradation pathway and strains used. (a) Intermediary products of the upper and lower parts of the metabolic degradation of atrazine, as well as genes associated with the different reactions are indicated. (b) The four ancestral strains, as well as their corresponding atrazine degrading gene repertoire are indicated. Genes encoding enzymes involved in the formation of N-containing by-products are coloured in blue while genes encoding enzymes involved in the formation of C-containing by-products are contoured.