Skip to main content
. 2019 Dec 4;5:148. doi: 10.1038/s41420-019-0229-8

Fig. 5. Differential regulation of TRAF proteins by mCD40L in normal (HRPT) and malignant (RCC) cells.

Fig. 5

RCC lines ACHN, 786-O and A-704, and normal HRPT cells were treated with mCD40L for the indicated time periods (1.5, 3 and 6 h) and expression of TRAF1 (a), TRAF2 (b) and TRAF3 (c) was detected by immunoblotting (40 µg protein/lane) in controls (‘C’) vs. mCD40L-treated cells (‘mL’). Equal loading for human epithelial cell lysate was confirmed by CK18 detection (see Methods). As positive controls for TRAF1 (a), TRAF2 (b) and TRAF3 (c) protein expression induction, in RCC cell immunoblotting experiments (top panels) lysates from HCT116 cells that were treated with control (‘C’) or treated with mCD40L (‘mL’) for 6 h were included, while for HRPT cell experiments (bottom panels) lysates from ACHN cells untreated or treated with mCD40L for 6 h were used. Lysate from effector (3T3CD40L) cells alone (20 µg protein/lane) served as negative control (NC) and confirmed the human-protein specificity of the anti-TRAF1 (a), anti-TRAF2 (b) and anti-TRAF3 (c) antibodies.