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. 2019 Dec 4;10:5518. doi: 10.1038/s41467-019-13520-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a SILAC-L/M/H-labeled A549 cells were infected with the human seasonal H3N2 IAV isolate Panama (Pan) or the avian H3N2 isolate Mallard (Mal) or left uninfected. The methionine analogous AHA was given to the methionine-depleted medium in different 4 h intervals. b After lysis and enrichment for proteins that incorporated AHA, samples were subjected to shotgun proteomics. Absolute and relative protein synthesis profiles were quantified for host and viral proteins. c iBAQ-based quantification of protein synthesis levels for host and viral proteins in cells infected with either Pan or Mal virus, as indicated. Median, 25th and 75th percentile of the respective populations are given. d Quantification of the total newly synthesized protein mass for host and viral proteins with either Pan or Mal infection as indicated. Data were normalized to the 0–4 h time period. All data are based on the mean of n = 2 biological replicates.