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. 2019 Dec 4;10:5516. doi: 10.1038/s41467-019-13543-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a–c PIN2:ubq:VEN in root tips of eir1-4 PIN2p::PIN2:ubq:VEN (a) and det2-1 eir1-4 PIN2p::PIN2:ubq:VEN grown in the absence (b) or presence of 100 nM eBL at 6 DAG (c). d PIN2:ubq:VEN signal ratios between PM and intracellular space in eir1-4 PIN2p::PIN2:ubq:VEN and det2-1 eir1-4 PIN2p::PIN2:ubq:VEN. 31-32 root epidermis cells in five roots were tested for each dataset. Relevant p-values obtained by One-way ANOVA with post-hoc Tukey HSD test are indicated. e, f PIN2:ubq:VEN distribution and abundance in 6 DAG eir1-4 PIN2p::PIN2:ubq:VEN root epidermis cells incubated on PNS (e) or after 16 h incubation on 10 μM Brassinazole (BRZ; f). g Western blots of eir1-4 PIN2p::PIN2:ubq:VEN membrane protein fraction, probed with anti-PIN2. Samples were incubated for 16 h in the presence of 100 nM 24-epiBL, 10 μM BRZ, or solvents only (PNS). h Western blots of eir1-4 PIN2p::PIN2:ubq:VEN (top panel) and bri1-6 eir1-4 PIN2p::PIN2:ubq:VEN (bottom panel) membrane protein fraction, probed with anti-PIN2. Samples were incubated for 16 h in the presence of 100 nM 24-epiBL, 30 μM bikinin, or on solvents only (PNS). i–n PIN2:ubq:VEN in root tips of eir1-4 PIN2p::PIN2:ubq:VEN (i–k) and bri1-6 eir1-4 PIN2p::PIN2:ubq:VEN (l–n) on PNS (i, l), or after incubation on 100 nM eBL (j, m) or 30 μM bikinin (k, n) for 16 h. o PIN2:ubq:VEN signal ratios between PM and intracellular space in eir1-4 PIN2p::PIN2:ubq:VEN and bri1-6 eir1-4 PIN2p::PIN2:ubq:VEN. 31-32 root meristem epidermis cells in 6 roots were tested for each dataset. Relevant p-values obtained by One-way ANOVA with post-hoc Tukey HSD test are indicated. Anti-α-tubulin (TUB) was used as loading control for Western blots. Whiskers in box plots cover the entire range of outliers obtained in the datasets; gray boxes: first and third quartiles; center line: median; dots: values obtained. Scale bars: a–c = 50 μm; e,f,i–n = 10 μm. Source data are provided as Source Data file.