Fig. 5. Crosstalk between brassinolide and PIN₂ in directional root growth.

a Deviation of root growth from vertical of 6 DAG Col0 and eir1-4 germinated in the presence of indicated eBL concentrations. 30-38 roots were analyzed for each dataset. b–e Vertically oriented 6 DAG Col0 (b, d) and eir1-4 (c, e) seedlings germinated on PNS (b, c) or 10 nM eBL (d, e). f Root reorientation of 6 DAG Col0 primary roots incubated on the indicated growth medium. 24-51 roots were analyzed for each dataset. g, h 6 DAG Col0 seedlings, after o/n gravistimulation on PNS (g) and on 100 nM eBL (h). White arrowheads indicate root overbending. i Root reorientation of 6 DAG eir1-4 primary roots incubated on the indicated growth medium. 24–47 roots were analyzed for each dataset. One-way ANOVA with post-hoc Tukey HSD test was used to determine p-values. ‘a’ indicates significant differences (p < 0.01) to Col0 control roots. Whiskers in box plots cover the entire range of outliers obtained in the datasets; gray boxes display first and third quartiles; center line: median; dots: values obtained. Scale bars: b–e, g, h = 10 mm. Source data are provided as Source Data file.