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. 2019 Dec 4;10(12):909. doi: 10.1038/s41419-019-2152-6

Fig. 3. Elevated ALKBH1 level in leukocytes and endothelium reduced m6A DNA in vitro.

Fig. 3

Human umbilical vein endothelial cells (HUVECs) and human monocytic cells (THP-1) were treated with various concentrations of oxidized low-density lipoprotein (ox-LDL) for 24 h. a–f Representative immunoblotting and quantification of N6AMT1 and ALKBH1 levels and global m6A level in ox-LDL–treated HUVECs (a–c) and THP-1 cells (d–f). GAPDH was the internal control. g qRT-PCR assay of ALKBH1 mRNA level with siRNA-Ctrl (Control) or siRNA-ALKBH1 transfection with or without ox-LDL (50 μg/ml) treatment in HUVECs and THP-1 cells. h ELISA analysis of global m6A level with ALKBH1 knock down in ox-LDL-treated HUVECs and THP-1 cells. Data are mean ± SD (n = 4/group) and were analyzed by one-way ANOVA, followed by Bonferroni’s multiple comparison test. *P < 0.05, **P < 0.01. ***P < 0.001 vs. 0 µg/ml ox-LDL. #P < 0.05 vs. 50 µg/ml ox-LDL for g and h.