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. 2019 Dec 4;10(12):909. doi: 10.1038/s41419-019-2152-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a–d Miat mRNA relative expression and correlation with ALKBH1 level in leukocytes and aortic root tissue in WD-induced AS mice (n = 4/group). e qRT-PCR assay of MIAT mRNA level with ox-LDL treatment in HUVECs. f, g Western blot assay of ox-LDL-induced ALKBH1 or HIF1α protein levels (f) and qRT-PCR assay of MIAT mRNA level (g) with siRNA targeting ALKBH1 or HIF1a pre-transfection in HUVECs. h qRT-PCR assay of MIAT mRNA level with ox-LDL treatment in THP1 cells. i, j Western blot assay of ox-LDL-induced ALKBH1 or HIF1α protein level (i) and qRT-PCR assay of MIAT mRNA level (j) with siRNA targeting ALKBH1 or HIF1a pre-transfection in THP1 cells. Data are mean ± SD (n = 4/group) and were analyzed by one-way ANOVA, followed by Bonferroni’s multiple comparison test. *P < 0.05, **P < 0.01. ***P < 0.001 vs. ND (a, c) or without ox-LDL treatment in (e, g, h and j).