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. 2019 Dec 4;10:5525. doi: 10.1038/s41467-019-13481-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Long-read sequencing analysis of the sgRNA targeted regions. a The two sgRNA target regions of MCPH1 (exon2 and exon4, respectively) tested by long-range PCR and PacBio sequencing. The upper and bottom panels show the coverage of the PacBio reads on the target regions based on whole-region view and cut-site region view, respectively. The cut sites of the sgRNAs in exon2 and exon4 are indicated with red arrow, red line, and shadow frame in red. b A ~300 bp insertion at the near-target region (2.3 kb from the cut-site) in two Cas9-edited monkeys (rmO1^ko and rmO4^ko*), which inherited from their wildtype mother (rmM1^wt and rmM2^wt).