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. 2019 Dec 4;10(12):917. doi: 10.1038/s41419-019-2156-2

Fig. 2. Sensitivity towards BH3-mimetics does not correlate with BCL-2 protein expression levels.

Fig. 2

a Expression levels of the main antiapoptotic (left) or proapoptotic (right) BCL-2 proteins in AML cell lines were analyzed by Western blotting. β-Actin and Vinculin served as loading control. Numbers below the Western blots indicate quantified expression levels of three independent experiments relative to loading control. b, c Selected AML cell lines were transiently transfected with siRNA against MCL-1 or non-targeting control siRNA (siCtrl). b Cell death was determined by externalization of phosphatidylserine (PS) and flow cytometry at 24 h after transfection in MOLM-13, MONO-MAC-6 or KASUMI-1 cells. Data are mean and standard deviation (SD) (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001. c MCL-1 expression was assessed by Western blotting with β-Actin serving as loading control.