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. 2019 Dec 4;10(12):917. doi: 10.1038/s41419-019-2156-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a MOLM-13 cells were treated with ABT-199 (1 μM) for indicated time prior to analysis of cell death by Annexin-V/FITC staining and flow cytometry. b MOLM-13 or MONO-MAC-6 cells were treated with S63845 (1 μM) for indicated time points prior to the analysis of apoptosis by Annexin-V/FITC staining and flow cytometry. c, d Caspase-3 cleavage was analyzed by Western blotting. Vinculin served as loading control. Arrowheads indicate cleavage fragments of caspase-3. e, f Loss of MMP was measured using TMRM staining and flow cytometry upon treatment with 1 μM of ABT-199 e or 1 μM of S63845 f–h. MOLM-13 or MONO-MAC-6 cells were treated with ABT-199 g or S63845 h for 4 h in the presence or absence of 50 μM of the pan-caspase inhibitor zVAD.fmk. Cell death was determined by Annexin-V/FITC staining and flow cytometry. Mean and SD of three independent experiments performed in triplicate are shown. **P < 0.01; ***P < 0.001.