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. 2019 Dec 4;10(12):917. doi: 10.1038/s41419-019-2156-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b MOLM-13 or MV4–11 cells were treated for 8 h with ABT-199 (1 μM) or for 4 h with S63845 (1 μM). MONO-MAC-6 cells were treated with S63845 (1 μM) for 2 h prior to analysis of BAX and BAK activation by IP. Input is shown to demonstrate equal protein expression in the lysate. A representative experiment is shown (n = 2–3). c, d MOLM-13, MV4-11 or MONO-MAC-6 cells were transiently transfected with siRNA against BAX or BAK or non-targeting control siRNA (siCtrl), followed by treatment with c ABT-199 (24 h) or d S63845 (MOLM-13 and MV4-11: 4 h, MONO-MAC-6: 2 h) and analysis of apoptosis by staining with Annexin-V/FITC and flow cytometry. Mean and SD of three independent experiments performed in triplicate are shown. *P < 0.05; **P < 0.01; ***P < 0.001. e Expression levels of BAX and BAK were assessed by Western blotting for transfected MOLM-13, MV4-11 and MONO-MAC-6 cells with GAPDH serving as loading control.