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. 2019 Dec 4;10:5524. doi: 10.1038/s41467-019-13500-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — The off-target assay for chimeric Cas12a-type variants. a The individual off-target assay. We designed nine different off-target spacers, of which three were substitutions, three were deletions, and three were insertions. b, c Genome-wide off-target analysis was done using the CIRCLE-seq method^56,57. b gRNA targeting the galK1 site and c gRNA targeting the lacZ2 site. Positions with mismatches to the target sequences, i.e., off-target sites, are highlighted in color. CIRCLE-seq read counts are shown to the right of the on- and off-target sequences and represent a measure of cleavage efficiency at a given site. The on/off-target reads shown in the figure were higher than 10. Source Data are available in the Source Data File.