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. 2019 Dec 4;10:5530. doi: 10.1038/s41467-019-13157-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Myc and NICD co-activation induces proliferation in adult mouse cochlea in vivo. a A diagram illustrating the procedure of ad-Myc/ad-Cre injection in adult Rosa-NICD cochlea (left). A diagram depicts injection into the scala media (SM) of adult cochlea by cochleostomy (middle). Enlarged inset of a cross section shows cochlear structure and cell subtypes (right). Cld: Claudius’ cells; HeC: Hensen cells; OHC: outer hair cells; IHC: inner hair cells; IDC: interdental cells; DC; Deiters’ cells; OPC: outer pillar cells; TC: tunnel of Corti; IPC: inner pillar cells; IPh: inner phalangeal cells; NF: neuro fibers of auditory neurons; and IBC: inner cells. b–i Four (b–e) and 35 (f–i) days after injecting ad-Myc/ad-Cre mixture to the adult (6-week-old) Rosa-NICD cochleae in vivo, proliferating IHCs (MYO7A⁺/BrdU⁺, arrows) and SCs (SOX2⁺/BrdU⁺, arrowheads) were detected. j–m Four days after control ad-V5 in vivo injection into the adult Rosa-NICD cochleae, no proliferating cell was detected. n, o Quantification and comparison of number and percentage of BrdU⁺ IHCs and SCs in adult Rosa-NICD cochleae infected with ad-Myc/ad-Cre in vivo for four days (n) or 35 days (o) in the mid-base turn, compared to the ad-V5-injected adult Rosa-NICD cochleae. ***p < 0.001, ****p < 0.0001, two-tailed unpaired Student’s t-test. Error bar, mean ± s.d.; n = 8 for ad-Myc/ad-Cre groups, n = 6 for control groups in (n); n = 6 for each group in (o). n is the number of biologically independent samples. Scale bars: 10 μm. Source data are provided as a Source Data file.