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. 2019 Dec 4;9:18299. doi: 10.1038/s41598-019-54840-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Influence of MSC. (A) Expression of Lipocalin-2 (Lcn2) significantly increased after DL (d0) and then gradually decreased to control level by d21 in both groups. (B,C) Western blot analysis of Lcn2. (B) Quantification of Lcn2 revealed a significant increase 5 min (d0), which decreased to control levels by d21 after saline and MSC injection. (C) Representative pictures of Lcn2 detection by western blot. (D) Representative pictures of STAT1α/β detection by western blot. (E) Expression of STAT1 slightly increased at d0 and d5. (F) Quantification of STAT1α revealed a slight increase at all time points after saline and MSC injection. (G) Quantification of STAT1β revealed a slight increase at all time points after saline and MSC injection. For western blot analysis the samples (n = 42) were run on four blots, which were processed in parallel. Full blots are provided in the supplementary data file. Data are n = 6, mean ± SD. *Represent p ≤ 0.05, ***represent p ≤ 0.001 and ****represent p ≤ 0.0001 compared to control.