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. 2019 Nov 28;10:2759. doi: 10.3389/fmicb.2019.02759

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Copyright © 2019 Wang, Zhang, Wang, Liu, Li, Ma, Yang, Zhang, Li and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Southern blotting verification of laeA, veA, and velB gene deletion. (A) The WT and ΔlaeA isolates were digested with EcoRI. A fragment amplified from ΔlaeA was used as the probe. (B) The WT and ΔveA isolates were digested with EcoRI. A fragment amplified from ΔveA was used as the probe. (C) The WT and ΔvelB isolates were digested with BamHI. A fragment amplified from ΔvelB was used as the probe.