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. 2019 Nov 28;10:1488. doi: 10.3389/fpls.2019.01488

Figure 3.

Figure 3

PYL10 regulates ABA content and genes for ABA metabolism. (A) PYL10 overexpression enhances ABA content in transgenic rice. (B–I) qRT-PCR gene expression of genes for ABA biosynthesis viz., OsZEP1, OsNCED1, OsNCED2, OsNCED3 and OsNCED5, ABA catabolism (OsABA8ox3) and ABA conjugation (OsBG2). Flag leaf sample from about 15 independent plants (biological samples) for each transgenic event were collected at flowering stage under control conditions. The leaves were cut in to small pieces and three replicates of about 1g each was sampled for three replicates for each transgenic event, and ABA was quantified by HPLC method. Part of the same flag leaf sample was used for gene expression analysis. The experiments were performed with three biological replicates. Values are mean (n = 3). Error bar indicates ±SE. Statistical differences are shown on the bars by labelling significantly different groups with different letters. *means P < 0.05.