Figure 4.

Cold stress tolerance of OsPYL10 overexpressing transgenic rice seedlings. (A) Photograph showing phenotype of seedling before and after stress and recovery. 25 days old seedlings were exposed to 15°C for 48 h (acclimation) followed by cold stress at 6°C for 48 h. These plants were recovered at normal temperature for one week and then photographs were taken. (B) Membrane stability at the end of cold stress treatment. (C, D) Seedling survival rate and total biomass after 7 days of cold stress recovery. The experiments were performed with three independent biological replicates (3 pots each with 20 seedlings). Value shown in graph are mean of biological replicates (n = 20). Error bar indicates ±SE. *means P < 0.05.