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. 2019 Nov 27;10:2740. doi: 10.3389/fimmu.2019.02740

Figure 2.

Figure 2

ERα deficiency decreased proliferation of Th17 cells. Th17 cells were differentiated for 3 days from WT female, WT male and Esr1−/− female mice and then restimlulated with PMA, ionomycin, and golgi-stop. (A) Representative flow gating of IL-17A cytokine expression in viable CD3+CD4+ T cells (Th17 cells). (B,C) Frequency and total numbers Th17 cells. (D) IL-17A mean fluorescent intensity (MFI) in Th17 cells. *p < 0.05 ANOVA with Tukey post-hoc analysis. Data was pooled from two independent experiments. n.s. means not statistically significant. (E,F) Th17 cell proliferation was measured using cell trace violet. (E) Representative histogram of cell trace violet staining on IL-17A+ CD4 cells 3 days after differentiation. (F) Proliferation index of Th17 cells. Data shown is from one of two experiments conducted. *p < 0.05 ANOVA with Tukey post-hoc analysis.