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IL-23R surface expression was decreased on Th17 cells from Esr1−/− mice. Naïve T cells were activated (Th0) and in select well-differentiated to become Th17 cells. (A) Il23r mRNA relative expression normalized to Gapdh. (B) Representative flow plots of IL-23R+ IL-17A+ CD3+ CD4+ viable T cells. (C) Total number of IL-23R+ IL-17A+ T cells. (D) MFI IL-23R in IL-23R+ IL-17A+ Th17 cells. *p < 0.05 ANOVA with Tukey post-hoc analysis. Data was pooled from two independent experiments.
