Figure 6.

Mitochondrial respiration and proton leakage were decreased in Th17 cells from Esr1^−/− mice. Th17 cells were differentiated from WT female, WT male and Esr1^−/− for 3 days and OCR was determined by Seahorse extracellular flux analysis. Representative plot of OCR (A) and ECAR (B) over time in Th17 cells with addition of oligomycin (1 μM), FCCP (1.5 μM) and Rot/AntA (0.5 μM). (C,D) Quantification of spare respiratory capacity and proton leak in Th17 cells. Data are taken from one representative experiment of the three independent experiments conducted. ^*p < 0.05, ANOVA with Tukey post-hoc analysis.