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. 2019 Nov 28;10:1492. doi: 10.3389/fpls.2019.01492

Figure 1.

Figure 1

Western blots assessing the activation of GCN2 via eIF2α phosphorylation. (A) Wildtype (Ler) and gcn2-1 plants were either treated 20 min with UV-C (control) or with white light supplemented for 1.5 h with 8 µmol m-2 s-1 broad band UV-B. Leaves were harvested immediately (imm.), 4 h and 6 h after UV-B shut down. (B) First signs of eIF2α phosphorylation (p-eIF2α) is detectable already 30 min after the onset of 10 µmol m-2 s-1 broad UV-B. (C) Also 1.5 h of filtered broad band UV-B exposure is activating GCN2. Equal amount of protein (20 μg) was loaded on 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis. LC, Loading control.