Location of MnSoxE1 gene detected by in situ hybridization. a Histological section of ovary at different ovary stages of M. nipponense. OC oocyte, N nucleus, CM cytoplasmic membrane, Y yolk granule, FC follicle cell, FM follicle membrane. b Histological section of vas deferens of M. nipponense. SC spermatocyte, E epithelial cell. CM cytoplasmic membrane, S sperm, c histological section of testis of M. nipponense. CT collecting tissue, SG spermatogonium, SE sertoli cells, SC spermatocyte, SC1 primary spermatocyte, SC2 second spermatocyte, ST spermatid, S sperm