Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 3;13(5):832–846. doi: 10.1016/j.stemcr.2019.09.002

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Iron-Induced Senescence in NF Fibroblasts

(A and B) (A) Cells morphology (upper panel) and SA-β-Gal activity (lower panel) in bright field. Scale bars, 20 μm. (B) SA-β-Gal activity of NF1 cells treated with iron (Fe) and NAC. Scale bars, 20 μm. Positive stained cells in (A) and (B) were counted and plotted as a percentage of the total cells. The results are presented as the mean ± SD of five fields in three independent experiments.

(C) Ultrastructural analysis of fibroblasts examined by EM evidencing vacuoles (white arrows) and mitochondria (black arrows).

(D) Immunoblotting of LC3I and II expression in soluble fibroblast homogenates. Results are presented as the mean ± SD of three independent experiments.

(E) Levels of IL-1β and IL-6 determined by ELISAs on cell media. The results are presented as the mean ± SD of three independent experiments in octuplicate. The data were analyzed by unpaired, two-tailed t test, except for (B) in which one-way ANOVA were used, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.