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. 2019 Oct 3;13(5):832–846. doi: 10.1016/j.stemcr.2019.09.002

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Iron-Induced Senescence in NF iPSC-Derived NPCs and Neurons

(A and B) (A) SA-β-Gal activity in NPCs. Scale bars, 20 μm. (B) SA-β-Gal activity in neurons. Scale bars, 20 μm. Positive stained cells in (A) and (B) were counted and plotted as a percentage of the total cells. The results are presented as the mean ± SD of five fields in three independent experiments.

(C) Immunoblotting of p62 expression in soluble neuron homogenates. Load represents Coomassie blue staining. Results are presented as the mean ± SD of three independent experiments. The data were analyzed by unpaired, two-tailed t test, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.