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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Cancer Metastasis Rev. 2019 Sep;38(3):445–454. doi: 10.1007/s10555-019-09810-8

Fig. 4.

Fig. 4

A single CAF exerts a pulling effect on a 3D DCIS structure as has been observed in other types of cancers. Prior to seeding, CAFs were labeled with CellTrace CFSE (green). Cultures were imaged live. Confocal image stacks, obtained on a Zeiss LSM-510 confocal microscope, were rendered in 3D with Volocity software. Prior to imaging, nuclei were labeled with Hoechst 33342. One grid is 45 μm