Figure 2.
Knowledge of carbohydrate biosynthetic pathways in bacteria provides important information on precursors for metabolic oligosaccharide engineering (MOE).
(a) Pseudaminic and legionaminic acid biosynthesis via three-carbon extension (PEP – shown in red) of hexose precursors via the Pse (Helicobacter pylori) and Leg (Campylobacter jejuni) pathways. (b) Azide-labeled MOE precursors 1, 2, and 3 for Pse, Leg and Kdo pathways (green box). (c) Octulosonic acid biosynthesis (E. coli) via a three-carbon extension (PEP – shown in red) of a pentose precursor. (d) Cellular uptake and fate of 1 and 3 in bacterial MOE studies.