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. 2019 Dec 4;39(12):BSR20192414. doi: 10.1042/BSR20192414

Figure 5. RISP gene knockdown blocks hypoxia-, caffeine- or NE- induced ROS production in PASMCs.

Figure 5

(A) Western blots of RISP expression in PASMCs uninfected, infected with lentiviral RISP shRNA, and non-silencing (NS) shRNA. (B) Mitochondrial and Cytosolic ROS (C) were determined after hypoxia treatment. RISP knockdown inhibited hypoxia-induced ROS increase. Data were obtained from three separate experiments. **P < 0.01 compared with normoxia NS_shRNA group, and #P < 0.05; ##P < 0.01 compared with hypoxia NS_shRNA groups. (D) RISP KO PASMCs were transfected with RISP overexpression plasmids. All groups were treated with hypoxia for 5 min. Mitochondrial ROS generation were measured using a Mitochondrial ROS Detection Assay Kit. **P < 0.01 compared with WT group, and #P < 0.05 compared with WT RISP KO group. (E) PASMCs were treated with caffeine (200 μM) (E andF) or NE (20 μM) (G and H) for 5 min in non-infected, infected with non-silencing (NS) shRNA PASMCs or lentiviral encoding RISP shRNA. Mitochondria and Cytosolic ROS were determined after treatment. Data were obtained from three separate experiments. ***P < 0.001 compared with non-infected control group, and #P < 0.05; ##P < 0.01 compared with caffeine-treated NS_shRNA group.