Figure 3.

Deficiency in rps9 induces anemia in mutant embryos. (A-B) Detection of hemoglobin levels by o-dianisidine staining for rps9 in both siblings and mutants at 48 hpf and 3 dpf. (C-E, E’) Detection of erythroid cells by crossing rps9 mutant into LCR:EGFP transgenic fish line. At 24 hpf, the number of blood cells are reduced slightly; At 48 hpf and 3 dpf, erythrocytes are almost deprived in the trunk, and only a small amount of red blood cells remain in the cardiac region. (F) Wright-Giemsa staining of erythroid cells at 3 dpf in sibling and mutant.