Figure 3.

Knockdown of Sec71 caused a reduction of both apical and basolateral proteins. RNAi line BDSC 32366 (Sec71-RNAi) was used. Flies with Sec71-RNAi were crossed to UAS-dicer-2; pGMR-Gal4. UAS-dicer-2/+; pGMR-Gal4/+ was used as control. Scale bars are 5 μm. (A) TLI does not reveal individual rhabdomeres in Sec71 knockdown PRCs (Class II defect). (B) Sec71 deficient PRCs show a severe reduction of Rh1, Crb, and Eys. (C) TEM shows a loss of IRS, missing rhabdomeres and gaps between ommatidial units of Sec71 deficient PRCs. (D) The K⁺Na⁺ATPase subunit Nrv is reduced in Sec71 knockdown PRCs. Acti-stain555 (F-actin) was used to visualize the rhabdomeres. (E) Summary model suggesting that Sec71 acts in the Golgi to control secretion of apical and basolateral factors. See Figure 1A for annotation and text for discussion.