Figure 2.

Regeneration Rosetta app identifies process-specific analysis after optic nerve injury. (A) Temporal transcript profiles of genes in the cholesterol metabolic pathway. Relative transcript counts from retinas dissected 2-, 4-, 7- and 12-days post injury (dpi) were compared with those from uninjured animals (0 dpi). Transcript expression is presented as TPM Z-scores; putative SREBF2 target genes are indicated to the right of the heatmap (biosynthetic enzymes in black; transcription factors are in red). (B) Specific enrichment of cholesterol metabolic and biosynthetic genes early in regeneration. Fisher’s exact test of over-representation was used to identify cholesterol-related GO-terms correlated with specific stages of regeneration.