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. 2019 Dec 4;4(6):e00814-19. doi: 10.1128/mSphere.00814-19

FIG 2.

FIG 2

LTA structure. (A) Proposed structure of S. gordonii type I LTA with glycerol phosphate (X) and terminal glucose-glycerol phosphate (Y) repeat units. (B) NMR spectrum of purified LTA extracted from S. gordonii containing two distinct anomeric proton signals. (C) Purified S. gordonii LTA was also subjected to electrospray ionization-mass spectrometry after monomerization by hydrofluoric acid. Multiply modified glycerol signals are observed at m/z 326.15, 348.14, 439.14, and 488.20. (D) Carbohydrate composition analysis of monomerized LTA by GC/MS (carbohydrate composition) identified glucose as the sole monosaccharide residue in the S. gordonii LTA, comprising 43.2% (mol%) versus 56.8% of glycerol.