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Multipotential differentiation of USCs. (a) SMC-differentiated cells showed an increased expression of the SMC-specific markers desmin, myosin, ASMA, and vimentin. (b) The UC-differentiated cells showed an increased expression of the UC-specific markers UP-Ia, UP-III, AE1/AE3, and CK13. NC: negative control; ASMA: alpha-smooth muscle actin; UP-Ia: uroplakin 1A; UP-III: uroplakin 3; AE1/AE3: cytokeratin, clone AE1/AE3; CK13: cytokeratin 13. Scale bar: 25 μm.
