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. 2019 Nov 22;2019:4748312. doi: 10.1155/2019/4748312

Figure 3.

Figure 3

Effects of MK0677 on mRNA expression of selected genes in WPMY-1 cells. (a) Effects of MK0677 (24 h, 100 nM) on mRNA levels of genes selected from microarray in WPMY-1 cells, assessed by RT-PCR. Shown are relative expression levels compared to unstimulated controls (normalized to mean of controls, which is 1) (single values of stimulated samples, from each of five independent experiments, and mean for each gene as bar). (b) Effects of MK0677 (24 h, 100 nM) on mRNA levels of proliferation markers in WPMY-1 cells, assessed by RT-PCR. Shown are relative expression levels compared to unstimulated controls (normalized to mean of controls, which is 1) (single values of stimulated samples, from each of five independent experiments, and mean for each gene as bar). (c) Ct values for GAPDH in cells with and without (control) stimulation with MK0677 (24 h, 100 nM) (single values of samples from each of five independent experiments, with paired samples from each independent experiment connected by a line).