Figure 3. LINC00115 physically associates with miR‐200s.

• A
  The prediction for miR‐200 binding sites in the LINC00115 transcript sequence.
• B, C
  MS2‐RIP followed by qRT–PCR analysis of endogenous microRNA association with LINC00115. 12XMS2 empty vector, LINC00115‐12XMS2, or LINC00115‐mut‐12XMS2 with Flag‐tagged MS2 was co‐transfected into 1123 GSCs.
• D
  Schematics of the predicted binding sites of miR‐200s on LINC00115 and LINC00115 mutants.
• E
  RNA pull‐down and qRT–PCR assays of LINC00115 binding with miR‐200s. GSC1123 cell lysates were incubated with biotin‐labeled LINC00115.
• F
  Luciferase activity analysis of 1123 GSCs co‐transfected with miR‐200s and luciferase reporters containing LINC00115 WT, mutant, or empty vector (EV).

Data information: In (C, E, and F), data are representative of three independent experiments. Error bars, ± SD. In (C), ***P < 0.001, by two‐tailed t‐test. In (E and F), *P < 0.05, **P < 0.01, by one‐way ANOVA.