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. 2019 Oct 21;20(12):e48109. doi: 10.15252/embr.201948109

Figure 1. CASP11 contributes to MRSA‐induced inflammasome activation in murine macrophages.

Figure 1

  1. Immunoblot analysis of CASP11 from WT, casp11 −/−, and casp1 −/− BMDMs infected with MRSA (MOI 5:1) at 24 h post‐infection. Densitometry analysis represents mean ± SEM (n = 3 biological replicates). Statistical analysis was performed using two‐way ANOVA. **P ≤ 0.01, NT = no treatment.
  2. Immunoblot analysis of cleaved CASP1 and IL‐1β in supernatants from WT, casp11 −/−, gsdmd −/−, casp1 −/−, and mlkl −/− BMDMs infected with MRSA (MOI 20:1) at 24 h post‐infection. Densitometry analysis represents mean ± SEM (n = 5 biological replicates). Statistical analysis was performed using two‐way ANOVA. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, NT = no treatment.
  3. Cytokine release from WT, casp11 −/−, gsdmd −/−, casp1 −/−, and mlkl −/− BMDMs infected with MRSA (MOI 20:1) at 24 h post‐infection. Data represent mean ± SEM (n = 8 biological replicates). Statistical analysis was performed using one‐way ANOVA. **P ≤ 0.01.