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. 2019 Oct 21;20(12):e48109. doi: 10.15252/embr.201948109

Figure EV2. Role of inhibitors affecting mtROS production, autophagy, and F‐actin polymerization on MRSA growth and macrophage viability.

Figure EV2

  1. MRSA growth in TSB in the presence of Ant A, FCCP, Cyto D, BafA1, rapamycin, and wortmannin (n = 3 biological replicates). Statistical analysis was performed using two‐way ANOVA. *P ≤ 0.05, ***P ≤ 0.001, NT = no treatment.
  2. MRSA growth in TSB in the presence of NAC and MitoQ (n = 9 biological replicates). Statistical analysis was performed using two‐way ANOVA. NT = no treatment.
  3. MRSA‐induced LDH release in supernatants from WT and casp11 −/− BMDMs (MOI 5:1) treated with Ant A or Cyto D at 24 h post‐infection (n = 8 biological replicates). Statistical analysis was performed using two‐way ANOVA. **P ≤ 0.01.
  4. Extracellular growth of MRSA in cell culture supernatants from WT and casp11 −/− BMDMs (MOI 5:1) treated with Ant A or Cyto D at 24 h post‐infection (n = 8 biological replicates). Statistical analysis was performed using two‐way ANOVA. NT = no treatment.
  5. Intracellular CFU of MRSA in WT and casp11 −/− macrophages treated with MitoQ at 24 h post‐infection (MOI 5:1). Data represent mean ± SEM (n = 8 biological replicates). Statistical analysis was performed using two‐way ANOVA. ***P ≤ 0.001, NT = no treatment.