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. 2019 Oct 2;20(12):e48297. doi: 10.15252/embr.201948297

Figure 1. ATF7IP represses the SETDB1‐regulated exogenous provirus reporter and ERVs in mESCs.

Figure 1

  • A
    Atf7ip KO cells show increased expression of SETDB1‐regulated ERVs and the provirus reporter, MSCV‐GFP (Fig EV1B), as evidenced by RT–qPCR analysis. RNA expression was normalized to Hprt expression and is shown relative to the level in WT cells. Data are mean ± SD; n = 3, technical replicates.
  • B
    Atf7ip KO mESCs (TT#2‐12) show decreased H3K9me3 at the LTR of the SETDB1‐regulated reporter and the ERVs, as evidenced by Native ChIP followed by qPCR analysis. Gapdh was used as a negative control. Data are mean ± SEM; n = 5, independent experiments. *P < 0.05 and **P < 0.01 by paired t‐test.
  • C
    SETDB1 cellular amount is mostly maintained in Atf7ip KO mESCs. Endogenous SETDB1‐ATF7IP interaction is validated by anti‐ATF7IP antibody co‐IP experiment with anti‐ATF7IP antibody.

Source data are available online for this figure.