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. 2019 Oct 2;20(12):e48297. doi: 10.15252/embr.201948297

Figure EV1. Establishment and characterization of Atf7ip KO mESCs (related to Fig 1).

Figure EV1

  • A
    The structure of MSCV‐GFP reporter construct. PBS: primer binding site.
  • B
    Establishment of Atf7ip KO cell lines by CRISPR/Cas9 technology.
  • C
    Confirmation of a complete loss of ATF7IP protein and comparable expression of SETDB1 in the parental WT and established Atf7ip KO cell lines, TT#2‐15 and TT#2‐12, by WB analysis.
  • D
    Flow cytometric analysis shows that Atf7ip KO cell lines increase the expression of MSCV‐GFP reporter.

Source data are available online for this figure.