Impact of bortezomib treatment on cellular protein stability. Same number of WT or
ATF7IP KO HEK293T cells was aliquoted into the 1.5‐ml tubes and untreated or treated with LMB (10 ng/ml) or/and bortezomib (100 nM) for 5 h before analysis which is same condition as used for Fig
3E. Ponceau‐S staining of same blotted membrane used for poly‐Ub and SETDB1 detection, respectively. The representative data of two independent experiments are shown.