A–ELocalization of Drp1, MFN2, Mcl1, MiD49, and MFN1 following CCCP treatment in Parkin‐expressing cells. In (B–D), HeLa cells stably expressing Parkin were transfected with 3Flag‐MFN2, 3HA‐Mcl1, or MiD49‐3HA plasmids. After 3 h of CCCP treatment, the cells were immunostained with anti‐Flag, anti‐HA, anti‐Tom20, and anti‐catalase antibodies. For Drp1 (A) and MFN1 (E), plasmids were not transfected. HeLa cells stably expressing Parkin were treated with 10 μM CCCP for 3 h and then subjected to immunocytochemistry using anti‐Drp1, anti‐MFN1, anti‐Tom20, and anti‐Pex14 antibodies. These previously reported substrates of MITOL do not localize to peroxisomes upon mitochondrial depolarization. Higher magnification images of the boxed regions are shown in the lower panel. Scale bars, 10 μm.
