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. 2019 Oct 10;20(12):e47728. doi: 10.15252/embr.201947728

Figure EV4. The intracellular localization of various MITOL mutants.

Figure EV4

As compared to K268, ubiquitylation of MITOL K40 and K54 was not critical for translocation from mitochondria to peroxisomes during Parkin‐mediated mitophagy. The K40A and K40R mutations had little inhibitory effect on peroxisomal redistribution of MITOL following CCCP treatment. The K54A and K54R mutations slightly impeded the migration of MITOL to peroxisomes, whereas the effect was weaker than K268A and K268R mutations. HeLa cells stably expressing HA‐Parkin were transfected with 3Flag‐MITOL wild‐type, K40A, K40R, K54A, or K54R mutants, treated with 10 μM CCCP for 3 h, and then subjected to immunocytochemistry with anti‐Flag, anti‐catalase, and anti‐Hsp60 antibodies. Scale bars, 10 μm.