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. 2019 Oct 10;20(12):e47728. doi: 10.15252/embr.201947728

Figure EV5. Retention of MITOL on depolarized mitochondria does not inhibit mitophagy progression.

Figure EV5

  • A
    Mitophagy progression in the presence of wild‐type MITOL or the translocation‐defective K268A MITOL mutant was monitored as a proportion of cells in which mt‐Keima underwent an acidification‐specific fluorescence change. FACS analysis revealed that the mitophagic flux in MITOL −/− HCT116 cells complemented with the MITOL K268A mutant was equivalent to that in MITOL −/− HCT116 cells complemented with MITOL wild‐type following 10 μM antimycin and 4 μM oligomycin for 3 and 6 h.
  • B
    Quantification of mitophagic flux. Error bars represent the mean ± s.d. in three biological replicates. Statistical significance was calculated using a one‐tailed Welch's t‐test.